Líquen Plano Oral e seu Potencial de Malignização / Lichen Planus and its potencial for malignancy

Objetivos: Instruir e orientar ao cirurgião dentista e demais profissionais de saúde a importância da detecção e rastreio precoce de lesões pré-malignas. Revisão de Literatura: O Líquen Plano Oral é uma condição dermatológica crônica, de origem auto-imune, relativamente comum na população, que atinge o epitélio de mucosa e pele, sendo considerada, pela Organização Mundial de Saúde (OMS), uma desordem potencialmente maligna quando associado a áreas de ulceração. A revisão de literatura foi realizada nas bases de dados PubMed e Lilacs. Buscamos investigar o potencial de malignização do Líquen Plano Oral associado a condições erosivas, analisando o processo de carcinogênese no processo inflamatório. Conclusão: Conclui-se que o objeto de estudo ainda é um assunto pouco explorado pela literatura, porém há indícios etiopatológicos que enfatizam o processo de malignização oriundo de uma lesão pré-maligna como o Líquen Plano Oral. Além disso, enfatizamos a importância do diagnóstico precoce das lesões estomatognáticas, para que assim possamos aumentar as chances de cura do paciente.(AU)

Objectives: To instruct and guide dentists and other health professionals on the importance of early detection and screening of pre-malignant lesions. Literature Review: Oral Lichen Planus is a chronic dermatological condition, of autoimmune origin, relatively common in the population, which affects the epithelium of the mucosa and skin, being considered, by the World Health Organization (WHO), a potentially fatal disorder. malignant when associated with areas of ulceration. A literature review was performed on the PubMed and Lilacs databases. We sought to investigate the potential for malignancy of Oral Lichen Planus associated with erosive conditions, analyzing the process of carcinogenesis in the inflammatory process. Conclusion: It is concluded that the object of study is still a subject little explored in the literature, but there are etiopathological accusations that emphasize the process of malignancy arising from a pre-malignant lesion such as Oral Lichen Planus. In addition, we emphasize the importance of early diagnosis of stomatognathic lesions, so that we can increase the patient's chances of cure.(AU)

Quality of Life in Patients with Chronic Oral Mucosal Conditions: A Qualitative Research

ABSTRACT Objective: To use qualitative research methods to evaluate the experiences of patients with chronic oral diseases. Material and Methods: Purposive sampling was used to recruit patients from the Oral Medicine Unit of Kerman University Dental School. An experienced independent facilitator convened the focus groups and conducted individual interviews in a non-clinical setting. Focus groups were mixed regarding their gender, age, chronic oral mucosal condition, time since diagnosis, and severity. A total of 39 patients participated in the study, including patients with oral lichen planus, pemphigus, recurrent aphthous stomatitis and leukoplakia. Results: Analysis of the interviews revealed that patient views could be divided into the following themes: biopsychosocial issues, treatment limitations and side effects, unpredictability of the conditions and the potential for malignant transformation. Chronic oral mucosal conditions affected patients' daily lives in various areas, from physical health and functioning to concerns about their future. Conclusion: The oral medicine practitioner's role in treating patients with chronic oral mucosal diseases extended beyond active management and symptomatic relief to the management of all aspects of these conditions that affected their daily lives.

YouTube™ and oral lichen planus: an appraisal of the educational quality of information

Abstract: The objective of this study was to analyze the quality of videos on oral lichen planus (OLP) available in YouTube™. A cross-sectional analytical study was conducted by searching the video sharing platform YouTube™. Videos aimed at clarifying the etiological and clinical characteristics, diagnostic criteria, treatment, and prognosis of OLP were included. A total of 481 videos were retrieved and 37 were included in the study according to the selection criteria established. Most of the videos evaluated (86.5%; n = 32) were produced by independent users. The average reliability was 1.8 and quality assessment classified only three videos (8.1%) as having good/excellent quality. A significant correlation was observed between the length of the video analyzed and its quality and reliability (p < 0.05), as well as between the reliability and usefulness of the video (p = 0.03). YouTube™ has become a leading source of information for the general population. However, a significant number of these videos have a low quality. Students, professionals, and healthcare providers must be more actively involved in providing clear, accurate, and reliable evidence-based information in an accessible language in order to enable significant improvement in patient care delivery.

Evaluation of serum and salivary transforming growth factor beta, vascular endothelial growth factor and tumor necrosis factor alpha in oral lichen planus / Evaluación sérica y salival del factor de crecimiento transformante beta, factor de crecimiento endotelial vascular y factor de necrosis tumoral alfa en liquen plano oral

Introduction: Lichen planus is one of the most common oral mucosal lesions. Transforming growth factor-ß (TGF- ß) has a marked effect on epithelial­mesenchymal transition and immune cells function. Vascular Endothelial Growth Factor (VEGF) is a key regulator of vasculogenesis and angiogenesis. Tumor necrosis factor-α (TNF-α) mediates T-lymphocyte homing and apoptosis of epithelial cells. Objetive: The present study was conducted in order to compare the expression of serum and salivary TGF- ß, VEGF, TNF-α between OLP patients and control individuals to investigate if saliva can be used as an alternative to serum for diagnostic purposes and for monitoring disease. Materials and Methods: 23 OLP patients and 23 control individuals were included to evaluate serum and salivary TGF-ß, VEGF, TNF-α using ELISA kits. Five milliliters of venous blood was collected and unstimulated saliva was collected by the spitting method. Results: Serum and salivary levels of TGF- ß, VEGF, TNF-α are higher in OLP patients compared to normal controls. Mean difference is higher in saliva than serum. Moreover, there was a significant difference in serum and salivary VEGF and TNF-α between symptomatic and asymptomatic groups. Conclusions: Saliva can be a used as a substitute for serum to evaluate levels of the assessed biomarkers.

Introducción: El liquen plano oral es una de las lesiones de la mucosa oral más comunes. El factor de crecimiento transformante ß (TGF-ß) tiene un efecto marcado sobre la transición epitelial-mesenquimal y la función de las células inmunes. El factor de crecimiento endotelial vascular (VEGF) es un regulador clave de la vasculogénesis y la angiogénesis. El factor de necrosis tumoral α (TNF-α) media la localización de los linfocitos T y la apoptosis de las células epiteliales. Objetivo: El presente estudio se realizó con el fin de comparar la expresión en suero y saliva de TGF-ß, VEGF, TNF-α entre pacientes con OLP y personas de control para investigar si la saliva se puede utilizar como alternativa al suero para fines de diagnóstico y monitoreo de la enfermedad. Material y Métodos: Se incluyeron 23 pacientes con OLP y 23 individuos control para evaluar los niéveles en suero y en saliva de TGF- ß, VEGF, TNF-α utilizando kits ELISA. Se recogieron cinco mililitros de sangre venosa y se recogió saliva no estimulada por el método de escupir. Resultado: Los niveles séricos y salivales de TGF-ß, VEGF, TNF-α son más altos en pacientes con OLP en comparación con los controles normales. La diferencia media es mayor en saliva que en suero. Además, hubo una diferencia significativa de VEGF y TNF-α en suero y saliva entre los grupos sintomáticos y asintomáticos. Conclusion: La saliva puede usarse como un sustituto del suero para evaluar los niveles de los biomarcadores estudiados

Salivary Transferrin Levels in Patients with Oral Lichen Planus

Objective: To compare salivary transferrin levels between patients with oral lichen planus (OLP) and healthy subjects. Material and Methods: In this descriptive, analytical, crosssectional study, 11 patients with OLP and 22 healthy subjects were selected after matching in terms of age and gender. OLP was confirmed by two oral medicine specialists based on clinical and histopathological criteria. Salivary samples were collected by spitting. The patients were asked to collect their saliva in their oral cavity and then evacuate it into sterilized Falcon tubes. The procedure was repeated every 60 seconds for 5-15 minutes. A total of 5 mL of saliva was collected using this method. The samples were collected from 8 to 9 in the morning in a fasting state to avoid circadian changes. The collected salivary samples were immediately placed next to ice and transferred to the laboratory to be centrifuged at 4°C at 800 g to isolate squamous cells and cellular debris. Then the samples were frozen at -80°C until the samples were prepared. An ELISA kit was used to determine salivary transferrin levels. Data were analyzed with descriptive statistics (means and standard deviations) and t-test for independent groups using SPSS 17. Statistical significance was set at p<0.05. Results: The mean salivary transferrin concentrations in patients with OLP and healthy subjects were 0.9055±0.28229 and 1.5932±0.80041 mg/dL, respectively (p<0.05). Conclusion: The salivary transferrin levels in patients with OLP were significantly lower than those in healthy subjects.

Cytokines, cortisol, and nitric oxide as salivary biomarkers in oral lichen planus: a systematic review

Abstract The etiopathogenesis of oral lichen planus (OLP) is still not fully elucidated, and it is believed that its development could involve a neuro-immune-endocrine profile. This systematic review investigated the relationship between cytokines, cortisol, and nitric oxide (NO) in the saliva of OLP patients. An electronic search was conducted in Pubmed/Medline, Scopus, LIVIVO, and Web of Science databases with no restriction of language to identify studies published up to December 2017. Data extraction was performed using the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines. A total of 140 articles were retrieved, and 32 articles fulfilled the inclusion criteria (cytokines = 17; cortisol = 9; NO = 6). The most studied cytokines in the saliva of OLP patients were interleukins IL-4, IL-6, IL-8, IFN-ү, and TNF-α, which were higher in OLP patients than in healthy controls (HC). Salivary cortisol was found to be higher in OLP than in HC in most (55.5%) of the selected studies, and all studies related to NO found higher levels of this marker in OLP than in HC. Despite controversial results, our review suggests that OLP patients have an increased inflammatory response, as indicated by the proinflammatory profile of salivary cytokines. In addition, we conclude that salivary cytokine and NO measurements may have significant diagnostic and prognostic potential for monitoring disease activity and therapeutic responses in OLP.

Liquen plano bucal. Presentación de un caso / Oral lichen planus. Presentation of a case

El liquen plano es una enfermedad inflamatoria, eruptiva de la piel, pruriginosa, de marcha crónica o subaguda que puede involucrar las mucosas, sobre todo la bucal. Su etiología es desconocida, se relaciona con estímulos subjetivos (estados nerviosos, depresivos). Se mencionan también como posibles causas: las infecciones, los trastornos metabólicos y endocrinos, recientemente se ha considerado que la enfermedad puede presentar una reacción de hipersensibilidad retardada. El caso clínico del presente trabajo fue una paciente de 54 años de edad, remitida por su médico de familia con antecedentes de alteraciones psicosomáticas, resultado de un estrés mantenido durante un período de tiempo prolongado. Presentaba lesión en zona de carrillos derecho e izquierdo y en cara dorsal de lengua. Se interpretó como lesión inflamatoria no infecciosa de la mucosa bucal, la cual fue biopsiadas, lo que trajo como resultado el desarrollo de un liquen plano bucal. El diagnóstico fue corroborado por el examen histopatológico. A la paciente se le realizó tratamiento en diferentes etapas con ansiolíticos e inmuno moduladores, obteniéndose resultados satisfactorios.

Lichen planus is a pruriginous, eruptive inflammatory disease of the skin, of chronic or sub acute course that may involve mucosa, mainly the oral one. Its etiology is unknown, and is related with subjective stimuli (nervous, depressive status). Infections, metabolic and endocrine disorders are also mentioned as possible causes. Recently it has been considered that this disease may present a retarded hypersensibility reaction. The current work deals with the clinical case of a female patient, aged 54 years, who was referred by her family physician with antecedents of psychosomatic disorders, as a result of stress maintained during a long time period. She had a lesion in the area of the right and left cheeks, and in the dorsal side of the tongue. It was taken as a non infectious, inflammatory lesion of the oral mucosa, from which a biopsy was obtained, resulting in the development of an oral lichen planus. The diagnosis was corroborated by histopathological examination. The patient was treated in different stages with anxiolitics and immunomodulators, achieving satisfactory results.

Oral lichen planus: clinical profile and determination of oral epithelial dysplasia / Liquen plano oral: perfil clínico y determinación de la displasia epitelial oral

Oral lichen planus (OLP) is the most common noninfectious oral disease and is considered a potentially malignant disorders (OPMDs). The etiology and pathogenesis of OLP are not completely understood and the malignant transformation remains under discussion. The aim was to asses the cytological and histological features of OLP, and establish relationship between clinical and microscopic profiles. A total of 10 patients diagnosed with OLP were investigated. Slides were prepared and stained with Hematoxylin­Eosin to identify 8 histological features and 8 cytological alterations. Finally, oral epithelial dysplasia degree was determined. Hyperplasia and loss of polarity of basal cells were detected in 90 % of cases. Anisonucleosis, nuclear pleomorphism and hyperchromasia affected 100 % of the sample. Six cases were classified as mild-grade OED, with no cases of severe-grade. Microscopic alterations were higher in women (P <0.0001) and moderate-grade OED was diagnosed only in this group (P <0.0014). The findings demonstrated that microscopic and clinical data association should be analyzed to a better understand of disease behavior. OED was absence only in one case, so the periodic follow-up of patients diagnosed with OLP is mandatory to avoid the malignant transformation.

El liquen plano oral (LPO) es la enfermedad no infecciosa más común y se considera un trastorno potencialmente maligno. La etiología y la patogénesis del LPO no se conoce y la transformación maligna sigue siendo objeto de debate. El objetivo de este estudio fue evaluar las características citológicas e histológicas del LPO y establecer la relación entre los perfiles clínicos y microscópicos. Un total de 10 pacientes diagnosticados con LPO fueron investigados. Secciones histológicas se prepararon y tiñeron con hematoxilina-eosina para identificar 8 características histológicas y 8 alteraciones citológicas. Finalmente, se determinó el grado de displasia epitelial. La hiperplasia y la pérdida de la polaridad de las células basales se detectaron en el 90 % de los casos. Anisonucleosis, pleomorfismo nuclear e hipercromasia afectaron al 100 % de las muestras. Seis casos fueron clasificados como displasia epitelial oral (DEO) de grado leve y no se reportaron casos de grado severo. Las alteraciones microscópicas fueron mayores en las mujeres (p <0,0001) y DEO de grado moderado fue diagnosticado sólo en este grupo (p <0,0014). Los resultados demostraron que la asociación de datos microscópicos y clínicos deben ser analizados para una mejor comprensión del comportamiento de la enfermedad. La DEO estuvo ausente sólo en un caso, por lo que el seguimiento periódico de los pacientes diagnosticados con LPO es necesario para evitar la transformación maligna.

Liquen oral, patrones reaccionales liquenoides y cáncer oral: estudio clínico e histopatológico retrospectivo de 12 años / Oral lichen, lichenoid reaction patterns and oral cancer: retrospective clinical and histopathological study of 12 years

Antecedentes: el liquen plano es una enfermedad con probabilidad de transformación maligna. La afectación oral se presenta en el 60 al 70% y el riesgo de cáncer oral es de 0-12,5%. La distinción entre liquen y reacción liquenoide oral es dificultosa. Se han descripto numerosos criterios para distinguirlas y se han asociado a riesgo de transformación maligna, pero aún así hay varias inconsistencias. Objetivo: determinar las características epidemiológicas y anatomopatológicas de los pacientes con liquen y reacción liquenoide oral. Establecer la frecuencia de progresión a cáncer considerando si hay diferencia entre los grupos. Diseño: estudio descriptivo, retrospectivo, observacional y longitudinal. Métodos: se recopilaron datos de pacientes evaluados en el Hospital Lagomaggiore de Mendoza, desde enero de 2003 a diciembre de 2014. Se incluyeron pacientes mayores de 18 años con confirmación histopatológica de liquen o reacción liquenoide oral. El análisis se realizó con Graph Pad Instat y se utilizaron medidas de tendencia central, de dispersión, test de normalidad y test exacto de Fisher. Resultados: se incluyeron 110 pacientes con confirmación histopatológica. Cuatro pacientes desarrollaron cáncer oral, 4,23% de los líquenes y 2,56% de las reacciones liquenoides. No hubo diferencia significativa entre grupos. En todos los casos, el cáncer oral ocurrió sobre la zona afectada previamente. Conclusiones: se registraron cuatro pacientes con progresión a cáncer oral. Estos hallazgos justifican el tratamiento y seguimiento. Se requieren más estudios para determinar si el liquen oral y las reacciones liquenoides son entidades separadas (AU)

Background: lichen planus is a disease with probability to become malignant. Oral affection occurs between 60% and 70% and the risk of oral cancer is from 0% to 12.5%. The difference between lichen and lichenoid oral reaction is troublesome. The literature has described numerous criteria to differentiate them and they have been associated to the risk of a malignant transformation, but there are still many inconsistencies. Objetive: to determine epidemiological and anatomopathological characteristics in patients with lichen and lichenoid oral reactions. To settle the frequency of progression to cancer, putting under consideration if there are differences between each group. Study design: Descriptive, retrospective, observational and longitudinal study. Methods: data have been taken from evaluated patients at Lagomaggiore Hospital in Mendoza, from January 2003 until December 2014. Patients over 18 years old with histopathological diagnosis of lichen or lichenoid oral reactions were included. The analysis was done with Graph Pad Instat and there were used central trends, dispersal, normal test and exact Fisher's test measures. Results: we include 110 patients with histopathological diagnosis confirmed. Four patients developed oral cancer; 4.23% of patients with lichen and 2.56% of patients with lichenoid reactions. We did not find a significant difference between groups. In all cases oral cancer occurs in the previously affected zone. Conclusions: there were registered four patients with cancer progression. These findings justify the follow up and treatment. More studies are needed to establish if oral lichen and oral lichenoid lesions are different entities (AU)

Contribution à l'étude des manifestations buccales du lichen plan

Introduction:Le lichen plan buccal est une dermatose inflammatoire chronique, Il touche d'avantage la peau et la muqueuse orale mais les phanères et les autres muqueuses peuvent être également touchées. L'objet de notre étude est de préciser les caractéristiques cliniques de la pathologie dans sa localisation buccale. Patients et méthode Le recrutement des patients s'est fait au service de pathologie bucco-dentaire du CHU Mustapha Ont été retenus les patients présentant une lésion évoquant cliniquement un lichen plan buccal. Tous les patients ont bénéficié d'un examen clinique, complété pour certains par un examen anatomo-pathologique. La saisie des données est faite sur le logiciel Epi Info 6.0 fr. Résultats 80 patients ont été recrutés entre Janvier 2009 et Juin 2015, Le taux de prévalence estimé de la maladie est de 0,11%. La pathologie est plus fréquente chez l'adulte entre 40 et 60 ans, avec une prédominance féminine. 63,75% des patients présentent des comorbidités notamment diabète (29,41%), HTA (47,05%), et 55% prennent un traitement médical de façon permanente et chronique, Le stress et l'état psychologique du patient ont été retenus comme facteurs déclenchant la maladie et exacerbant la symptomatologie. La forme réticulée est plus fréquente (78,75%) suivie par la forme érosive (53,75%), L'atteinte est dans 84% des cas bilatérale, La localisation jugale prédomine (88,75%), Le traitement médical reste à effet palliatif, nous avons noté un cas de transformation maligne (1,44%), chez une femme après 03 ans d'évolution d'un lichen plan érosif lingual. Conclusion Le lichen plan est une pathologie auto-immune chronique, dans sa localisation buccale pose un problème de diagnostic, le traitement médical reste palliatif, l'élimination des facteurs irritatifs locaux et la prise en charge psychologique des patients permettent de mieux gérer les symptômes et les poussées aigues. Le risque de transformation maligne existe et par conséquent un suivi régulier du malade s'impose

Potencial de transformación maligna del liquen plano oral: estudio retrospectivo / Potential malignant transformation of oral lichen planus: retrospective study

El liquen plano oral (LPO) es una enfermedad mucocutánea inflamatoria más común en las mujeres. Su etiología aún no es bien definida y esta condición puede estar relacionada, por ejemplo, a la ansiedad, estrés, diabetes, las enfermedades autoinmunes, infecciones y predisposición genética. El LPO se manifiesta en dos formas principales: reticular y erosivo. El objetivo de esta investigación es, a través de un estudio retrospectivo, analizar el posible potencial maligno del LPO. Se analizaron los registros de los pacientes atendidos en dos proyectos de lesiones bucales de dos diferentes instituciones en el período de 1995 a 2014. Se recogieron datos como género, edad, presencia de enfermedad sistémica, presencia de lesiones en la piel, tabaquismo, duración de la lesión, localización, síntomas, tratamiento, proservación y transformación maligna. Los resultados mostraron que, de los 3488 analizados, 85 (2,4%) tuvieron un diagnóstico confirmado de LPO, que afecta fundamentalmente a pacientes de sexo femenino (85%) siendo el LP reticular más común. En relación a la transformación maligna del LPO, 1 paciente (0,85%) tenía carcinoma de células escamosas 4 años después del diagnóstico inicial de LPO. Además, 3 pacientes mostraron atipia celular moderada en el diagnóstico. Los resultados refuerzan el potencial de transformación maligna del LPO. Aunque esta asociación es especulativa, la posibilidad debe ser considerada por los profesionales, que deben presentar estos pacientes a largos periodos de preservación.

Oral lichen planus (OLP) is an inflammatory mucocutaneous disease, more common in females. Its etiology is not yet well defined; this condition may be related, for example, to anxiety, stress, diabetes, autoimmune diseases, infections and genetic predisposition. The OLP manifests itself in two main forms: reticular and erosive. This work aims to perform a retrospective study analyzing the possible malignant potential of OLP. Records of the patients were analyzed in two projects of oral lesions in two different institutions in the period from 1995 to 2014. Data as gender, age, presence of systemic disease, presence of skin lesions, smoking, duration of the injury, location, symptoms, treatment, follow-up time and malignant transformation were collected. The results showed that, of the 3488 analyzed, 85 (2.4%) had a confirmed diagnosis of OLP, which mainly affected female patients (85%) being the most common the reticular LP. Regarding the malignant transformation of OLP, one patient (0.85%) had squamous cell carcinoma 4 years after the initial diagnosis of OLP. In addition, 3 patients showed moderate cellular atypia. The results reinforce the potential of malignant transformation of OLP. Although this association is speculative, this possibility must be considered by the dental practitioners, who must submit these patients to long periods of monitoring.

Oral Atrophic Lichen Planus: Diagnosis and Management.

Lichen planus is a chronic T-cell mediated autoimmune disease, which affects the oral mucosa, skin, genital mucosa, scalp and nails. Oral lichen planus, the mucosal counterpart affects 0.5-2.0% of the general population and often seen in the fifth to sixth decades of life. The lesion is frequently seen in women. Since it is considered a potentially malignant disorder, early diagnosis with timely management and regular follow-up is very important to avoid further complications. This paper reports a case of atrophic lichen planus, highlights the classic picture of atrophic lichen planus, discusses the differential diagnoses and timely management and follow-up.

Líquen plano oral: reconhecendo a doença e suas características básicas / Oral lichen planus: recognizing thedisease and its basic feature

Introdução: o Líquen plano é uma doença mucocutânea comum, crônica e inflamatória. A Organização Mundial da Saúde classificou o líquen plano como uma doença cancerizável mas de difícil identificação dos agentes etiológicos. Contudo, o consumo de bebidas alcoólicas, tabaco, fatores genéticos, agentes infecciosos e imunológicos devem ser levados em consideração no potencial de malignização. Objetivo: a proposição deste trabalho é relatar uma revisão literária atualizada sobre o Líquen Plano Oral (LPO) focada em bases de dados nacionais e internacionais (Pubmed, Bireme). Busca de forma sucinta, explicitar o conceito, dados epidemiológicos, características clínicas e histopatológicas do LPO, bem como seu potencial de carcinogênese e formas de tratamento. Revisão de literatura: todo cirurgião dentista deve ter conhecimento das diferentes formas de líquen plano oral, procurando reconhecer os aspectos lesionais que indicam alteração de normalidade, diagnosticar corretamente as patologias e buscar um tratamento adequado ao paciente portador dessa e outras lesões. Considerações finais: a forma erosiva do LPO é considerada a única que tem potencial de carcinogênese, sendo o diagnóstico precoce e o tratamento adequado essenciais para o sucesso do tratamento. -

Introduction: The Lichen planus is a common mucocutaneous disease, chronic and inflammatory. The World Health Organization ranked the lichen planus as a disease that can turn malignant in about 2-3 per cent but it is difficult to identify the etiologic agents. However, alcohol consumption, tobacco, genetic, infectious and immunological agents should be taken into account in the malignant potential. Objective: the purpose of this work, from a literature review on the topic of oral lichen planus (OLP) focused on bases of national and international databases (Pubmed, BIREME). Succinctly get the concept, epidemiological data, clinical features and histopathology of OLP) and its potential for carcinogenesis and its forms of treatment. Literature review: emphasizing that every dentist should have knowledge of different forms of oral lichen planus, seeking to recognize the lesional aspects that indicate change of normality, correctly diagnosing the disease and seeking a suitable for your patient with this treatment and other injuries. Final considerations: The erosive form of OLP is the only one that has potential for carcinogenesis, with early diagnosis and proper treatment essential for successful treatment.

Lichen Planus in Four Clinical Forms.

Lichen planus is an autoimmune disorder which comes under lichenoid reactions. It is T-cell mediated cytotoxic reaction directed against antigen expressed by basal cell layer of the oral mucosa. Autoreactive T-lymphocytes may be of primary importance for the development of oral lichen planus. Lichen planus presentations in the oral cavity are in 6 forms: Reticular, papular, plaque, bullous, erythematous and ulcerative. We present a case report of 4 forms in a patient.

Oral lichen planus: a look from diagnosis to treatment / Liquen plano oral: una mirada desde el diagnóstico al tratamiento

Oral lichen planus (OLP) is a chronic mucocutaneous disease of unknown etiology. Its pathogenesis is multifactorial and it may affect the oral mucosa, skin and other mucous membranes. Diagnosis is based on clinic and histopathology; direct immunofluorescence techniques can also be of use. It affects about one to two percent of the population, mainly women between the fifth and sixth decades of life. In the mouth, the most affected area is the buccal mucosa, followed by the gums, tongue and/or palate. Its three most representative clinical forms are reticular, erythematous and erosive; evolution depends on the type it is. Lesion treatment is determined by the clinical form and, since no fully effective treatment has been found yet, it is directed towards controlling the disease. The treatment of choice involves topical or systemic corticosteroids, but other drugs may also be used. The aim of this paper is to gather current and relevant information about oral lichen planus: its pathogenesis, diagnosis, treatment and management.

El liquen plano oral (LPO) es una enfermedad crónica mucocutánea de etiología poco conocida, cuya patogénesis es multifactorial, y puede afectar a la mucosa oral, piel y otras mucosas. El diagnóstico está basado en la clínica y la histopatología. Además, técnicas como la inmunofluorescencia directa pueden contribuir al diagnóstico de la enfermedad. Afecta del 1 al 2 por ciento de la población, principalmente mujeres, entre la quinta y sexta décadas de la vida. En boca, la zona más afectada es la mucosa yugal, seguido de encías, lengua y/o paladar. Las tres formas clínicas más representativas son: reticular, eritematosa y erosiva, cuya evolución varía según el tipo. El tratamiento de las lesiones depende de la forma clínica y está dirigido hacia un control de la enfermedad, ya que en la actualidad no se conoce un tratamiento del todo efectivo. El tratamiento de elección es la utilización de corticoides, en forma tópica o sistémica, aunque otros fármacos también pueden ser utilizados para el manejo de la enfermedad. El objetivo del presente artículo es recopilar información actualizada y relevante del liquen plano oral en su etiopatogenia, diagnóstico, tratamiento y manejo de la enfermedad.

Estudo da expressão de genes relacionados à resposta inflamatória e autoimune em lesões de líquen plano oral do tipo reticular por meio de PCR-array / Study of the expression of inflammatory response-related genes and autoimmune in lesions of oral lichen planus reticular type by PCR-array

O líquen plano oral (LPO) é uma doença inflamatória crônica que afeta em torno de 1 a 2% da população mundial adulta, entre 30 e 60 anos, principalmente mulheres. As lesões podem estar presentes em diversos sítios, porém tem predileção pela mucosa jugal, gengiva e bordas laterais da língua, todas bilateralmente. As manifestações de LPO são frequentes, e podem se caracterizar clinicamente em seis tipos: reticular, em placas, papular, atrófico, erosivo-ulcerativo e bolhoso, porém o mais comum é o reticular. O presente estudo teve como objetivo avaliar a expressão de genes relacionados à resposta inflamatória e autoimunidade no líquen plano oral (LPO) em pacientes com a variante exclusivamente reticular comparado a controle saudável. Foram incluídos consecutivamente 10 pacientes com LPO que preencheram os critérios de inclusão adotados, sendo 9 mulheres e um homem, e 6 indivíduos controle, sendo 4 mulheres e 2 homens pareados por sexo, idade e uso de medicações sistêmicas. Amostras de mucosa bucal foram coletadas por meio de biópsia incisional em ambos os grupos de pacientes e submetidas à extração de RNA para posterior análise da expressão gênica por PCR-array selecionada para este estudo. Os resultados obtidos foram o aumento da expressão de 8 genes, CXCL 9 e CXCL 10 vão de acordo com a literatura, corroborando com nosso estudo e de certa forma reafirmar mais necessidade de estudos bem desenhados...

The oral lichen planus (OLP) is a chronic inflammatory disease that affects around 1-2% of the adult population between 30 and 60 years, mainly women. The lesions may be present in several sites, but has a predilection for the buccal mucosa, gums and lateral borders of the tongue, all bilaterally. The manifestations of OLP are frequent, and may be characterized clinically in six types: reticular, plaque-like, papular, atrophic, erosive-ulcerative and bullous, but the most common is the reticular . The present study aimed to evaluate the expression of genes related to inflammatory response and autoimmunity in oral lichen planus (OLP) in patients with exclusively reticular variant compared to healthy control. 10 consecutive patients with OLP who met the inclusion criteria, 9 women and one man, and six control subjects were included were 4 women and 2 being proportionally matched by sex, age and use of systemic medications men. Samples of oral mucosa were collected by incisional biopsy in both groups of patients and subjected to RNA extraction for analysis of gene expression by selected for this study-PCR array. The results were the overexpression of genes 8, where only two of them go according to the literature, corroborating our study and somehow reaffirm need for more well-designed studies...

Diagnóstico diferencial das manifestações das sífilis e da Aids com líquen plano na boca: relato de caso / Differential diagnosis of manifestations of syphilis and Aids with lichen planus in mouth: case report

A sífilis segue um curso semiológico em que aparece, desaparece e ressurge com variados sinais e sintomas, exigindo dos examinadores conhecimentos clínicos e laboratoriais que os conduzam ao seu diagnóstico. A disseminação do Treponema pallidum envolve praticamente todos os sistemas orgânicos,dificultando diagnósticos e justificando a histórica consagração de que a sífilis é uma grande simuladora de várias outras doenças. A mucosa bucal é importante área sinalizadora para o reconhecimento da doença através de exame clínico sistemático e detalhado. Infelizmente, a incidência e prevalênciade sífilis no mundo não expressa sinais claros de pronunciada diminuição, sobretudo na América Latina e nos países ditos emergentes. O propósito dopresente trabalho é relatar o caso de um paciente com manifestações clínicas de sífilis e aids (síndrome da imunodeficiência adquirida) na boca, que foram primariamente interpretadas como líquen plano.

Syphilis follows a semiological course in which it appears, disappears, and reappears with various signs and symptoms, demanding clinical and laboratorial knowledge that lead to its diagnosis from examiners. The dissemination of Treponema pallidum involves practically all of the organic systems, complicating diagnosis and justifying the historic consecration that syphilis is a large simulator of various other diseases. The oral mucosa is an important signalizing areafor disease recognition by way of a systematic and detailed clinical examination. Unfortunately, the incidence and prevalence of syphilis in the world does notexpress clear signs of pronounced decrease, especially in Latin America and in the so-called emerging countries. The purpose of the present study is to report thecase of a patient with clinical oral manifestations of syphilis and aids (acquired immunodeficiency syndrome) that were primarily interpreted as lichen planus

Estudo de polimorfismos de nucleotídeo único em genes de receptores Toll-like em pacientes com líquen plano oral e pacientes com carcinoma epidermóide de boca / Toll-like receptor single nucleotide polymorphisms in patients with oral lichen planus and oral squamous cell carcinoma

Polimorfismos em genes de receptores Toll-like (TLR) podem modular o risco de desenvolvimento de infecção, inflamação crônica e câncer. O objetivo deste estudo foi investigar a associação de polimorfismos em TLR ao risco aumentado de desenvolvimento de câncer de cabeça e pescoço, o carcinoma epidermóide (CEC) de boca e de laringe, e lesões bucais com potencial de transformação maligna, como o líquen plano oral (LPO), incluindo lesões idiopáticas e lesões liquenóides (LLO). Para tal foi conduzido um estudo caso-controle com 40 pacientes com CEC de boca, 35 com CEC de laringe, 175 com LPO (129 idiopático e 46 LLO) e 89 controles saudáveis, todos de origem basca. Oito SNP nos TLR1, TLR2, TLR4, TLR6, TLR9 e TLR10 foram genotipados por ensaios TaqMan® ou pirosequenciamento. A análise estatística por meio do teste qui-quadrado mostrou que a variante A, para o SNP TLR2-rs4696480 aumentou significativamente o risco para o desenvolvimento de CEC de boca (p=0.03) e LLO (p=0.0223). O genótipo AT representa risco de desenvolvimento de CEC de boca aumentado em 5.3 vezes quando comparado ao genótipo TT (OR=5.3, IC95%=1.19-13.63), e genótipo AA em 6.6 vezes (OR=6.6, IC95%=1.30-33.89). Quanto ao desenvolvimento de LLO, o genótipo AT representa um aumento no risco de 4.6 vezes comparado ao genótipo TT (OR=4.6, IC95%=1.55-13.38) e o genótipo AA em 4.1 vezes (OR=4.1, IC95%=1.33-12.88). Embora os genótipos AT e AA ocorram com significativa frequência no grupo LPO idiopático (p=0.045), este SNP não foi correlacionado estatisticamente à susceptibilidade de desenvolvimento deste. O SNP TLR2-rs4696480 pode ser relevante para o risco de desenvolvimento de CEC de boca e LLO nesta população, incentivando novos estudos sobre a possível associação destes grupos de doenças e SNP no gene do TLR2, colaborando com a demonstração de polimorfismos de TLR como marcadores úteis do prognóstico e prevenção do câncer de boca.

Polymorphisms in toll-like receptor (TLR) genes may modulate the risk of infection, chronic inflammation and cancer. This study investigated whether TLR polymorphisms were associated with an increased risk of head and neck cancer, including oral (OSCC) and laryngeal squamous cell carcinoma (LSCC); and oral premalignant disorders such as oral lichenoid disease (OLD), including oral lichen planus (OLP) and oral lichenoid lesions (OLL). This case-control study included 40 OSCC, 35 LSCC, 175 OLD (129 OLP and 46 OLL) patients and 89 healthy controls, all of them from the Basque Country. Genetic polymorphisms in TLR1, TLR2, TLR4, TLR6, TLR9, and TLR10 were genotyped by TaqMan® assays or pyrosequencing. Chi-square analysis showed that the variant A for the SNP TLR2-rs4696480 increased OSCC (p=0.03) and OLL (p=0.0223) risk significantly. AT genotype increases the risk of developing an OSCC by 5.3 times compared with TT genotype (OR=5.3, 95%CI=1.19-13.63), and the AA by 6.6 times (OR=6.6, 95%CI=1.30-33.89). AT genotype increases the risk of developing OLL by 4.6 times compared with TT genotype (OR=4.6, 95%CI=1.55-13.38) and the AA by 4.1 times (OR=4.1, 95%CI=1.33-12.88). Although these mutated genotypes were significantly frequent in the OLP group (p=0.045), this SNP was not correlated with OLP susceptibility. TLR2-rs4696480 polymorphism may be relevant to OSCC and OLL susceptibility in this population; encouraging further studies to assess the possible association of this group of potentially malignant disorders and oral cancer with TLR2 SNP, which may help to demonstrate that TLR polymorphisms may be useful markers to prognosis and cancer prevention.

Papel da apoptose no infiltrado inflamatório do Líquen Plano Bucal / Inflammatory infiltrate of oral lichen planus of erosive and reticular types

O Liquen Plano Bucal (LPB) é uma doença inflamatória crônica auto-imune, cujas formas clínicas mais comuns são os tipos reticular e erosivo. A persistência do infiltrado inflamatório é importante para a patogênese do LPB, mas os mecanismos envolvidos neste processo ainda precisam ser mais onvestigados. A deficiência de apoptose pode contribuir para essa persistência do infiltrado subepitelial, que é composto predominantemente por linfócitos T CD4+ e CD8+. É sabido que o alvo das células CD8+ (LT citotóxicos) são ceratinócitos no LPB. O objetivo deste estudo foi avaliar se há bloqueio da apoptose nos linfócitos subepiteliais, elucidar as vias apoptóticas envolvidas, bem como quantificar os linfócitos T CD8+ nas lesões de LPB dos tipos reticular e erosivo...